- Performer:Anders Ek
- Title:The Phenol Red Solution
- Style:Prog-Rock,Alternative Pop/Rock,Alternative/Indie Rock
- Date of release:2003
- Recording date:2002 - 2003
- Size FLAC version1338 megabytes
- Size MP3 version1903 megabytes
- Size WMA version1366 megabytes
- Formats:RA VOC AIFF MPC ASF FLAC
The Phenol Red Solution. Anders Ek. The Phenol Red Solution.
On this page you can not listen to mp3 music free or download album or mp3 track to your PC, phone or tablet. All materials are provided for educational purposes. Released at: This album was released on the label Anders (catalog number A-00001).
Phenol red (also known as n or PSP) is a pH indicator frequently used in cell biology laboratories. Phenol red exists as a red crystal that is stable in air. Its solubility is . 7 grams per liter (g/l) in water and . g/l in ethanol. It is a weak acid with pKa . 0 at 20 °C (68 °F). A solution of phenol red is used as a pH indicator, often in cell culture.
Phenol Red, . 1% solution. Dissolve 10 mg of the reagent in 1 ml of . M NaOH and dilute the solution with water to 100 ml. Standard bromide solution: 1 mg/ml. Dissolve in water . 900 g of potassium bromide dried at 110 °C, and dilute the solution with water in a volumetric flask to 1 litre. Borate buffer, pH . –8. 8; saturated borax (Na2B4O7. Measure the absorbance of the solution at 580 nm against a blank solution.
A solution of phenol red will have a yellow color at a pH of . or below and a red color at a pH of . and above. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Phenol re. ompare this item. Phenol Red Indicator Solution. Standardized for colorimetric pH determination Color range: pH . -8. Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when growing cells at low densities.
The shelf life of liquid reagents is reduced by temporary contact with oxygen in the air when the bottle is opened as well as by unsuitable storage environments (presence of sunlight or high temperatures)
The problem with the phenol red is, it can absorb at the same wavelength as the MTT. So Washing the cells with PBS removes this problem. The solution is simple: either you use medium without phenol red for such assays, or you follow the protocol of Tada et al (or similar)- here the SDS-solution to dissolve the crystals contains . 1 HCl, making it acidic and when adding this to your cell medium any pH-dependent differences in phenol red color will be removed.